4 edition of Antisense and ribozyme methodology found in the catalog.
Includes bibliographical references and index.
|Statement||Ian Gibson, ed.|
|Contributions||Gibson, Ian, 1938-|
|LC Classifications||QP623.5.A58 A55 1997|
|The Physical Object|
|Pagination||xiii, 79 p. :|
|Number of Pages||79|
|LC Control Number||97016369|
Ribozyme Protocols (Methods in Molecular Biology) th Edition by Philip C. Turner (Editor) ISBN ISBN Why is ISBN important? ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book. Format: Hardcover. Nucleic acid based technology (antisense, ribozyme, DNA enzyme, RNAi) has made huge advances in the last few years and generated considerable expectations especially in the neurosciences. As a frontier method, these new technologies may be used to combat diseases, as well as to study gene functions by interfering in the genetic flow of Cited by: 1.
Ribozymes are being increasingly used for the sequence-specific inhibition of gene expression by the cleavage of mRNAs encoding proteins of interest. However, particular attention must be paid to the following points: the identification of regions on the mRNA accessible to the ribozyme; the delivery of ribozymes to cells by either exogenous or endogenous delivery; colocalization of the Cited by: The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use—^not only to those with some experience of ribozymes—^but also to those wishing to use ribozymes for the first time. Although it is usually impossible to cover every aspect of a scientific field.
Antisense Drug Technology book. Principles: Strategies, and Applications. Antisense Drug Technology. Analytical Methods for Antisense Drugs. View abstract. chapter 4 | 23 pages A Role for Antisense Technology in the Discovery of Highly Cited by: Antisense catalytic RNAs that specifically base-pair with and cleave target RNA sequences have potential for use as therapeutic agents against viral as well as endogenous gene expression. With the ultimate goal of developing anti-human immunodeficiency virus type 1 (HIV-1) ribozymes for therapeutic use, we have been exploring ways to improve Cited by:
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Antisense and ribozymes have a relatively short yet successful history as research tools in gene expression studies, and thus are considered as having high potential reagents in treating Antisense and ribozyme methodology book infections and cancer.
This laboratory companion provides detailed information on the potential, advantages and limitations of this methodology. Antisense–ribozyme technology has evolved from being a tool in molecular biology to being used as a therapeutic agent in human disease.
There is enormous potential in antisense and ribozyme therapy, but significant pitfalls and obstacles have yet to be overcome.
Download Citation | Antisense and Ribozyme Methodology: Laboratory Companion | Antisense and ribozymes have a relatively short yet successful history as research tools in gene expression studies. The ribozyme applications appearing in this book should benefit not only those experienced in ribozymes, but also those applying this ribozyme technology for the first time.
It is hoped that a better understanding of the therapeutic application ofribozymes will have a significant impact on human disease in the near : Hardcover. Antisense and ribozymes have a relatively short yet successful history as research tools in gene expression studies, and thus are considered as having high potential reagents in treating viral infections and laboratory companion provid.
Insertion of a mutant ribozyme, mainly capable of antisense activity, into ADDP cells resulted in smaller reductions in c-fos gene expression and in cisplatin resistance than the active ribozyme. "A wide variety of methods used to deliver genes to tumors are discussed, as are the type of genes used.
This includes tumor vaccination, suicide genes, antisense genes, ribozymes, etc. The unique aspect of this book is the translational approach of explaining laboratory techniques as well as the application to patients. Part of the Methods in Molecular Biology™ book series (MIMB, volume ) Castanotto, D., et al.
() Detection of antisense and ribozyme accessible sites on native mRNAs: application to NCOA3 mRNA. Mol. Ther. 4, – CrossRef PubMed Google Scholar. Le, S. and Zuker, M. () Predicting common foldings of homologous RNAs.
J Cited by: 1. Described in step-by-step fashion and enriched with each author's own practical tips, these readily reproducible methods are currently being widely applied in cancer gene therapy investigations, including immunotherapy and tumor vaccination, suicide gene therapy, antioncogene therapy, and antisense and ribozyme gene therapy.
Electronic books Laboratory manuals: Additional Physical Format: Print version: Antisense and ribozyme methodology. London ; New York: Chapman & Hall, © (DLC) (OCoLC) Material Type: Document, Internet resource: Document Type: Internet Resource, Computer File: All Authors / Contributors: Ian Gibson.
Part of the Methods in Molecular Medicine™ book series (MIMM, volume ) Log in to check access. Buy eBook. USD The authors show how antisense oligonucleotides are being designed and studied in relation to hypertension, various cancers, inflammatory bowel disease, brain disorders, the blood-brain barrier, and drug delivery.
Antisense oligodeoxynucleotide and ribozyme design. Probst JC(1). Author information: (1)Wilex Biotechnology GmbH, Grillparzerstrasse 10b, Munich, D, Germany. @ The overwhelming advances of the last few years in the field of nucleic acid-based technologies laid the basis for the development Cited by: This state-of-the-art reference provides comprehensive coverage of the development of antisense oligonucleotides to inhibit cancer cells as well as those involved in infectious, inflammatory, and immune-mediated diseases-highlighting new tools and technologies in medicinal chemistry, RNA biochemistry, and molecular and cellular biology to produce new therapeutic ts previously Reviews: 1.
Nucleic acids are the backbone of antisense therapy. Antisense oligonucleotide-based therapeutics involves downregulation of gene expression. RNA-based drugs that include antisense oligonucleotides bear great therapeutic potential toward treatment of various diseases by altering RNA and/or reducing, restoring, and modifying protein expression through multiple molecular : Shashwat Sharad.
Buy Therapeutic Applications of Ribozymes (Methods in Molecular Medicine) Softcover reprint of the original 1st ed. by Scanlon, Kevin J. (ISBN: ) from Amazon's Book Store. Everyday low prices and free delivery on eligible : Paperback.
Therapeutic Applications of Ribozymes contains all the practical detail necessary to realize the high promise of ribozyme technology as a significant, widely used methodology. Focused on ribozyme targets and the novel associated technology, the book immediately becomes the leading resource for all those seeking to transform this new category of Format: Tapa dura.
(For assistance in designing and applying ribozyme technology please refer to two recently published methods books: Ribozyme Protocols and Antisense and Ribozyme Methodology) As detailed above, ribozymes have been successfully used to target specific by: Ribozyme Protocols will help all investigators perform meaningful and successful experiments using ribozymes in both clinical and basic research, as well as launch fresh application initiatives.
It is certain to be found indispensable not only by those workers new to the field, but also by experienced researchers who wish to expand their range. Part of the Methods in Molecular Medicine™ book series (MIMM, volume ) Abstract Over a span of more than two decades, antisense strategies for gene therapy have expanded from antisense oligonucleotides (AS-ODNs) solely, to the addition of ribozymes and, more recently, to the inclusion of small interfering RNAs (siRNAs).
The oligonucleotide is considered antisense relative to the mRNA, and binding of the antisense oligonucleotide to the target mRNA hinders or eliminates translation of that mRNA. The effect of this action is decreased expression of the disease gene.
Second, ribozyme therapy is being developed to disable target mRNAs. Ribozymes are autocatalytic RNA molecules that cleave RNA targets. Books with RNA Protocols - RNA Methods Books. Biological Research Protocol Books >> Biochemistry and Molecular Biology >> Nucleic Acids >> RNA.
Definition of RNA - Ribonucleic acid (RNA) is one of two forms of nucleic acids and it is responsible for transferring information from the hereditary DNA into a form that is used and expressed by an organism.Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) with which it hybridizes, and thereby blocks its translation into protein.
asRNAs (which occur naturally) have been found in both prokaryotes and eukaryotes, antisense.